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대한생식의학회지 제26권 제3호 2010년
자궁내막증 환자의 복강액내 IGF가 자궁내막 기질세포 증식에 미치는 영향
서울대학교 의과대학 산부인과학교실
김정구, 서창석, 김석현, 최영민, 문신용, 이진용,
Effects of insulin-like growth factor in peritoneal fluid of patients with endometriosis on the proliferation of endometrial stromal cells
Kim jg, Suh cs, Kim sh, Choi ym, Moon sy, Lee jy
요약제목: 복강액내 IGF와 자궁내막 기질세포증식 The purposes of this study were to evaluate the effects of insulin-like growth factor(IGF)s in peritoneal fluid(PF) from patients with and without endometriosis on the proliferation of endometrial stromal cells and to investigate the effects of type I IGF receptor antibody on the response of endometrial stromal cells to PF from patients with endometriosis. IGFs in PF from patients with endometriosis(n=14) and without endometriosis(n=10) were measured by immunoradiometric assay and PF samples were divided into low IGF-I PF group(less than 85ng/ml) and high IGF-I PF group(more than 85ng/ml). Endometrial stromal cells from patients without endometriosis were cultured in serum free media in the presence or absence of 1% PF and thymidine incorporation test were used to evaluate the proliferation of endometrial stromal cells. Also cultures were incubated with type I IGF receptor monoclonal antibody( IR3) before adding PF. PF from patients with endometriosis and without endometriosis increased thymidine incorporation in endometrial stromal cells. In patients with endometriosis, high IGF-I PF group had high IGF-II levels and resulted in higher thymidine incorporation than low IGF-I PF group but no significant difference in increase in thymidine incorporation between high IGF-I and low IGF-I PF group was noted in patients without endometriosis. There was not a significant correlation between increase in thymidine incorporation and IGF-I levels in PF from patients without endometriosis but in PF from patients with endometriosis. Preincubation with IR3 significantly inhibited the mitogenic response of endometrial stromal cells to PF. Our data indicate that IGF-I in PF may be involved in the growth of ectopic endometrium in patients with endometriosis.
키워드 : Endometriosis, IGF, Peritoneal fluid, Endometrial stromal cells
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