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대한생식의학회지 제26권 제3호 2010년
착상기간의 자궁내 환경이 생쥐 난자 및 배아의 투명대 미세구조에 미치는 영향
한양대 자연과학대학 생명과학과1, 한양대 의과대학 해부학교실2, 을지병원 의과학연구소3, 을지대학교 의과대학 생리학교실4, 경기대학교 이과대학 생물학과5, 이화여자대학 의학과6
한성원13, 정호삼2, 강희규3, 이호준3,4, 계명찬5, 김성례6, 김문규1,
The Effect of Uterine Environment during Peri-implantation Period on the Ultrastructure of Zona Pellucida in Mouse Oocytes and Embryos
Han sw, Chung hs, Kang hg, Lee hj, Gye mc, Kim sr, Kim mk
In the studies on the hatching mechanisms in mammals, many investigators focused on the embryonic intrinsic factor(s) in in vitro culture, but the uterine environment as an extrinsic factor(s) is thought to play an important role in hatching mechanism. Therefore to evaluate the effect of uterine environment on the hatching event in vivo, the immature(GV) and ovulated (MⅡ) oocytes, and the late 2-cell embryos of mouse were transferred to pseudopregnant foster mother's uterus during peri-implantation period. So it was verified whether there would happen hatching by only uterine environment independently on embryonic stage. The ultrastructural changes of the zona surface of transferred group were compared with those of in vivo and vitro group by SEM. 36hrs after transfer, the immature and ovulated oocytes almost degenerated, and the late 2-cell embryos developed to various embryonic stages. However, the embryos which didn't develop to blastula stage did not hatch. The ultrastructural network of ZP in transferred group seemed to be smoothed uniformly, which was different from in vitro group. In conclusion, it is suggested that the uterine environment during peri-implantation period enhances the embryo hatching by provoking the structural change of ZP.
키워드 : Hatching, Mouse embryos, Zona pellucida (ZP), Uterus, SEM
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