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대한생식의학회지 제26권 제3호 2010년
난자내 정자 직접주입술에서 난자의 처리방법이 난자의 발생능력에 미치는 영향
경북대학교 의과대학 산부인과학교실, 대구대학교 자연자원대학 축산학과
박기상, 이택후, 송해범, 전상식,
The Effects of Oocyte Preparation on the Developing Capacity of Human Oocytes at Intracytoplasmic Sperm Injection (ICSI)
Park ks, Lee th, Song hb, Chun ss
Objective: In the preparation of ICSI, cumulus and corona cells should be removed from the oocytes by using a combination of enzymatic (hyaluronidase) and mechanical (pipetting) methods. But little is known about the effects of different degrees of oocyte denudation and incubation time between denudation and sperm injection on the outcomes of ICSI. The aim of this study was to evaluate the effects of varying the degrees of oocyte denudation and the lengths of incubation time from denudation to sperm injection on the outcomes of ICSI. Methods: In experiment 1, patients (oocytes) were grouped into group A and B according to the degree of denudation, complete and partial, respectively. In experiment 2, patients (oocytes) were grouped into group Ⅰ, Ⅱ and Ⅲ according to the length of incubation time of denuded oocytes until sperm injection as < 1, 1∼2 and >2 hours, respectively. Results: There was no significant difference between the degree of oocyte denudation on the survival, fertilization and development rates after ICSI procedure. In case of the incubation time of denuded oocytes until ICSI, survival rates was higher in group Ⅲ (83.1%) than in group Ⅰ (61.5%, P<0.05) or group Ⅱ (64.3%). However no statistically significant differences were found between incubation time and fertilization or development rates. Conclusions: This study reveals that the outcomes of ICSI are not affected by the degree (complete or partial) of oocyte denudation. However, the denuded oocytes with incubation period of more than 2 hours show better outcomes of ICSI than those with the incubation period of less than 2 hours.
키워드 : Degree of oocyte denudation, Incubation time, Intracytoplasmic sperm injection
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